BIOL308 Study Guide - Dnac, Phosphodiester Bond, Dna Polymerase I
31 Oct 2013
School
Department
Course
Professor
Document Summary
Study questions: lec 10-11: list proteins (enzymes and other factors) involved in the process of. Explain briefly the role of each of these proteins in replication. Dnab/helicase move along dsdna and separate strands (req atp) Dnaa initiates replication at oric by recognizing four dnaa boxes (9bps) only if the dna is negatively supercoiled. Dnac -clamp ladder required by dnab to escort it to dnaa forming the pre-priming complex. Single-stranded dna binding proteins (ssb) small proteins that bind to ssdna cooperatively and prevent double helix from reforming and forced. Functions with dnab to form next priming site (primosome) Dna pol iii key enzyme that catalyzes synthesis of dna from rna primers. Dna pol i removes ribonucleotides including those directly linked to the. Rnase h removes most the of the rna primers except the last one that is directly linked to dna.
