MBG 2040 Final: Chapter 10- DNA replication

• School: University of Guelph
• Department: Molecular Biology and Genetics
• Course: MBG 2040
• Professor: Baozhong Meng

Document Summary

Baltimore (rna transcribed back to dna) in retroviruses and retro- like viruses defied the central dogma. Monozygotic twins: accurate dna replication in action. The standard b-form dna helix of watson and crick. A:t g:c: a is bonded to t with only 2 h-bonds; it"s easier to break then the 3 h-bonds between c and g. Right-handed double helix (b form dna): the most common form in cells in living organism. Semi-conservative mechanism- parental molecule is half conserved. Dna are conserved and used as templated for synthesis of complementary segments that would subsequently be joined to produce progeny dna strands. Meselson and stahl proved that semi-conservative model was the correct copying mechanism for dna. Experimented on e. coli to demonstrate that chromosome replicates semi-conservatively. Replication begins from an origin , oric in e. coli. There is only one origin in the circular genome of e. coli-