BIOC 2580 Study Guide - Final Guide: Crispr, Glycosylation, Zinc Finger Nuclease
16 Dec 2015
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Can function in either homologous or non-homologous recombination. If used in non-homologous, it is usually to produce a deletion or a gene knockout (when dsb is introduced into exon) If used in homologous, it is usually to correct or introduce a specific gene sequence into a region by providing a dna templa te at the same time as the dsb is introduced. Fusion of zinc finger targeting domain and fok1 nuclease domain (type iis restriction enzyme-->cleaves outside of recognition sequence) Each zinc finger contacts 3 base pairs of dna. Multiple zinc fingers contacting different trios of base pairs can increase specificity. Two zinc fingers created with complementary base pairs for each dna strand so. Each repeat binds to one base based on which two amino acids are differing. Better than zinc fingers, but still not great because to change the target, the entire protein needs to be re- synthesized grna recognizes sequence, tracerrna recognizes repeats.
