BIOC 2580 Study Guide - Midterm Guide: Ribonuclease, Activation Energy, Collision Frequency

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: Enoka Wijekoon

Document Summary

To study long protein chains, they are cut into shorter oligopeptides by selective hydrolysis (divide and conquer) Selective hydrolysis cuts the polypeptide at specific locations, to yield a limited number of oligopeptides of definite size. The digestive enzyme trypsin binds and recognizes arg & lys side chains in peptides. Carboxylate group of the arg or lys is positioned next to catalytic unit of trypsin, and is target for hydrolysis of peptide bond. Trypsin and chymotrypsin convert polypeptides into smaller fragments. Except the fragment from the c-terminus (so can easily identify it) Chymotrypsin acts on to yield all fragments (except c-terminus) have phe/tyr/trp at the c-end. Cyanogen bromide is a chemical reagent, which cuts polypeptide chains at methionine residues. Cyanogen bromide br-c = n attacks s atom of met. Peptide chain is broken on carboxylate side and met is converted to homoserine, hse (serine with extra ch2) All fragments (except c-terminus) have hse at c-end.