BIOC 2580 Study Guide - Midterm Guide: Differential Centrifugation, Ammonia, Electrophoresis

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: Enoka Wijekoon

Document Summary

Proteins chains of amino acids: protein size is expressed in kilodaltons (kda) Myoglobin is a protein that stores oxygen in muscle tissue. A unique sequence of diff. amino acids. Each amino acid has an amino group and a carboxylate group. Each amino acid has a different side chain r. 20 different amino acids are found in proteins. Hydrolysis regenerates the original carboxylic acid and amino group. C=o group of the amide is the point of weakness allowing h2o attack. In order to break the bond, you add. Large numbers of amino acids can be linked together to form a peptide chain. Polypeptide a chain with many amino acids, usually a complete protein. Oligopeptide a chain with a few amino acids, usually a fragment. Peptide chain has a amino- terminal end (h3n+) and a carboxyl-terminal end (coo-) Carbon atoms of the amino acid core are identified by greek letters. The alpha- carbon is the central backbone atom.