BIOL-107 Study Guide - Photosystem, Chemiosmosis, Photophosphorylation
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Cell biology Questions
1. Where is the sugar-phosphate backbone located in a DNA strand within the DNA double helix?
a) Alternates between outside and inside
b)Inside
c)Outside
d)Varies between purines and pyrimidines
e)Depends on the species
2) Which of these statements defines paracrine cell signaling?
a) | A messenger molecule released into the blood stream to bind to a receptor on a cell far away from the release site. | ||||||||||||||||
b) | A messenger molecule released by a cell into the extracellular space that binds to receptors on the surface of the same cell. | ||||||||||||||||
c) | A messenger molecules released by a cell into the extracellular space that travels short distances to bind to a receptor in a nearby cell. | ||||||||||||||||
d) | A messenger molecule release by a tissue into the lumen of an organ that diffuse to bind to receptors far away from the release site. | ||||||||||||||||
e)All of the above. 3. Which effect has the binding of the ligand to a G-protein coupled receptor on intracellular G-protein?
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Experiment 2: Analysis of phosphate acquisition mutants of E. coli.
Present the table from the handout on which you recorded your results. (No marks, will be used as a guide for marking Q2) â¨
Strain | Growth on Pi plates(yes/no) | Growth on G3P plates (yes/no) | AP-ase assay colour |
1 | No | Yes | Yellow |
2 | No | Yes | None |
3 | Yes | Yes | Yellow |
4 | Yes | Yes | None |
Use your results to determine the genotype of each strain (bacteria are haploid, so you would use the terms â(loss of function) mutantâ or âwild typeâ) and complete the table below. (1 mark) â¨
Genotype | ||
Strain | Alkaline phosphatase | Pi transporter |
1 | ||
2 | ||
3 | ||
4 |
Explain how you reached your conclusions for the genotype of each strain. (1 mark) â¨
Why is growth on G3P is an important control for this experiment? (2 marks) â¨
Suppose the DNA sequences for the alkaline phosphatase and the Pi transporter genes from â¨strain 1 were determined. If both genes are found to contain a single base change compared to the wild type sequences, what could you conclude about the effect of each mutation on the encoded protein? (2 marks) â¨