FORS-2106EL Study Guide - Quiz Guide: Lysis Buffer, Restriction Enzyme, Endonuclease
27 Jun 2018
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FORS-2106 - Lab 7 Quiz Review
-DNA analysis can provide positive identification with great accuracy
-Restriction enzymes are endonuclease which catalyze the cleavage of the phosphate
bonds in a DNA chain
-they will generate a 5’ (phosphate) to 3’ (hydroxyl group) at the point of cleavage
-restriction enzymes are produced by many different species of bacteria
-FBI uses Hae III as a restriction enzyme
-to make agar:
-we used agarose powder and concentrated buffer and distilled water
-the garish had to be cooled down to 55 degrees celsius before added to the tray
-to make electrophoresis chamber buffer
-we used concentrated buffer, and distilled water
-we used GelGreen to stain the gel trays
-for genes is a bottle:
-we used lysis buffer after inserting cheek swabs
-then added used protease to our tubes
-then we used alcohol
-DNA precipitated out of our sample
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Document Summary
Dna analysis can provide positive identi cation with great accuracy. Restriction enzymes are endonuclease which catalyze the cleavage of the phosphate bonds in a dna chain. They will generate a 5" (phosphate) to 3" (hydroxyl group) at the point of cleavage. Restriction enzymes are produced by many different species of bacteria. Fbi uses hae iii as a restriction enzyme. We used agarose powder and concentrated buffer and distilled water. The garish had to be cooled down to 55 degrees celsius before added to the tray. We used concentrated buffer, and distilled water. We used gelgreen to stain the gel trays. We used lysis buffer after inserting cheek swabs. Then added used protease to our tubes.
