deepthikarthika2

Normal and Reversed phase are the two most common separation techniques applied in HPLC. Which of the following are true about normal phase separation? Choose all that apply

a) Non-Polar Compounds travel the fastest and elute first

b) Selectivity is function of particle size

c) The mobile phase is polar

d) C18 is used as a stationary phase

Each of the following statements describes a type of column chromatography.Sort the statements to the type of chromatography they describe. Ifa statement can describe all of the types, place that statement inthe "All" category. (Note that size-exclusion chromatography mayalso be called gel-filtration or molecular exclusionchromatography.)

Categories: Size-exclusion Chromatography, Affinity Chromatography,Ion-exchange Chromatogrpahy, and All

Statements:

Separate molecules by size.

Separate molecules by charge.

The stationary phase has a covalently bound group to which aprotein in the mobile phase can bind.

Uses a mobile phase and a stationary phase to separateproteins.

The stationary phase contains cross-linked polymers with differentpore sizes.

Can separate molecules based on protein-lignand binding.

The stationary phase may contain negatively or positively chargedgroups.

Name two types of chromatography that can also be considered to have a stationary phase and a mobile phase to allow the separation of the mixtures.

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