lima

lima

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lourdes limaWilliam E. Macaulay Honors College

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English1Biology4Finance3
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The mutagen 5-bromouracil (5BU) was added to a rapidly dividing culture of wild-type E. coli
cells growing in a liquid medium containing a rich variety of nutrients, including arginine. After
one cell division, the cells were washed free of the mutagen, resuspended in sterile water, and
plated onto master plates containing minimal medium supplemented only with arginine. Plates
were obtained having well-separated colonies, so that each colony derived from just one
progenitor cell. The colonies were then replica-plated from the master plates onto plates
containing minimal medium. One colony that grew in the presence of arginine but failed to grow
on minimal medium was selected from the master plate. The cells of this colony were
suspended in sterile water, and each of 20 tubes containing minimal medium supplemented
with arginine was inoculated with a few cells from this suspension. After the 20 cultures grew to
a density of 108

cells/mL, 0.1 mL from each was plated on plates containing minimal medium.
The following table shows the number of bacterial colonies that grew on each plate.

a. In which stage(s) of this process did mutations occur? What is the evidence that a mutational
event occurred?
b. At each stage where mutations occurred, were the mutations induced or spontaneous? Were
they forward or reverse mutations?
c. At each stage where mutations were recovered, how were they selected for?
d. Though all of the 20 cultures started from a single colony that failed to grow on minimal
medium were treated identically, they produced different numbers of bacterial colonies when
they were plated. Why did this occur?
e. Suppose that 5BU had been added to the medium in the 20 tubes. Would plating the 20
cultures have given the same results? If not, how would they have differed?
f. Supposing that methyl methane sulfonate (MMS) rather than 5BU had been added to the
medium in the 20 tubes, answer the questions given above in part (e).

Resposta:Explicação passo a passo: a) As mutações ocorreram durante a exposiçã...

Can you please do this biology assignment?

In this task you will find an article related to personal nutrition or nutrition of a society. Choose an article from a publication for the general public. There are lots of biology-related news articles, but remember you have to write a reflection about the one you choose, and your writing is always most effective and enjoyable to read if the topic strikes a chord with you. Choose something that interests you, not just the first article that pops up in your search. For this reason, controversies are usually easier to write about. You will show how the issue in the article relates to personal choices and/or choices in society about nutrition and food systems.
 
Your article must be:
  • published within the last year;
  • published by a reputable news source, ideally from North America;
  • printable (if online);
  • relevant to the topics we covered in this Unit.
Some possible sources:
  • Ottawa Citizen,
  • Globe & Mail,
  • National Post,
  • Toronto Sun,
  • Toronto Star,
  • Boston Herald
  • New York Times,
    The Economist,
    BBC,
    Scientific American.
     
    You will show your work in a written reflection.
    It is up to you how you organize your thoughts into paragraphs.
    Your written reflection should include the following:
    • A brief summary of the issue discussed in the article (max. 2 sentences)
    • How is this related to what we studied?
    • How is this relevant to personal choices or choices a government could make?
    • Any questions left unanswered
    • APA referencing for the article and any other sources of information you use
    These are very basic guidelines. How you write your reflection completely depends on what your article talks about. Feel free to make connections beyond the article to your life, any statistics you find, etc. The connections you make in your reflection to the course content and other relevant issues are much more important than your summary of the article.
Resposta:Explicação passo a passo:Eu posso tentar ajudá-lo com a tarefa de bio...

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