sarangnagaich

What is the botany

[25 points]

You have recently isolated and cloned the gene and cDNA for a novel

calcium binding protein from fruit fly muscle tissue. Initial comparisons of the sequence with

other similar proteins from Frog, Rat, and Cow indicate that this is a highly conserved protein.

Your major model system for studying the development and function of skeletal muscle is

mouse. Design and describe a series of experiments that will allow you to clone the mouse

cDNA and gene from mouse muscle tissue (remember you already have clones for fruit fly, frog,

rat, and cow). How could you determine the mRNA expression level of this gene in mouse

muscle?

Question 2) [25 points]Define Genomics Proteomics, and Bioinformatics and discuss how they are used for studying gene expressio

The goal of this group project is to develop a mouse model system for studying how mutations in the murine (mouse) IGF-1 gene might impact body size and lifespan in the mouse. (To learn about IGF-1 and how it functions to regulate growth, longevity, and cancer susceptibility, see the review I have posted in the discussion titled "Group Project Resources." Also in this discussion, you will find other resources related to molecular and cellular biology techniques.)

As part of this project, your goals are to:

Clone the mouse IGF-1 gene. You will need to discover how to isolate the IGF-1 gene from a mouse and place the gene into a vector for further analysis and use. Remember, you need to isolate the gene, not the mRNA/cDNA.

Characterize tissue expression patterns of mouse IGF-1 mRNA and protein. This section should explain how you will analyze tissue expression patterns in the wild type and knock out mice. These techniques are general techniques used to monitor mRNA and protein levels in the mice.

Introduce mutations into the cloned IGF-1 gene, and determine whether these mutations affect expression of the gene*. This section should explain how you plan to introduce mutations into your isolated IGF-1 gene. This mutated gene will then be used in the next step to create the knockin mice.

Generate an IGF-1 "knockin" mouse. Here, you will "knockin" your mutated IGF-1 gene into the mouse IGF-1 locus so that you can finally determine whether the mutations you have made in the gene will affect mouse size and lifespan.

*Any of the assays you work out to monitor expression of the normal mouse IGF-1 gene can then be used to assess expression of the mutated versions of the gene that you will be constructing.



During the course of the semester each group will post their progress on each goal (1 - 4 above) in the appropriate conference area. This will allow everyone to read and discuss the ideas in progress. This process is meant to enhance each groups project and come up with the best solutions to our challenge of characterizing IGF-1 in the normal mouse and the “knock-in” mouse.

1. How is genome engineering related to systems biology ?

2. List the methods used to isolate single cells, and explain how each method is conducted. Provide advantages and disadvantages of each method.

I need to collect information and to write a full description for the following components related to “Gelatin” topic in skin tissue engineering/ wound healing :

Swelling and degradation properties

Porosity

Scaffold fabrication methods

Advantages and disadvantages

Conclusion