Discuss how the cell is the fundamental unit of life
A.) A plasmid contains a multiple cloning site inside the coding region of the lacZ gene and also contains an ampicillin resistance gene at a separate locus. When cells are transformed with a successfully recombinant plasmid containing a piece of DNA cloned into the multiple cloning site, what color will the colonies be when grown on an agar plate containing ampicillin and X-gal?
(a.) green (b.) white (c.) colonies will not grow (d.) blue (e.) clear
B.) If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease identify the appropriate cut sites in the genome?
(a.) the endonuclease cuts randomly in the genome
(b.) the endonuclease recognizes the gene of interest
(c.) the endonuclease identifies its specific recognition sequence
(d.) the endonuclease cannot identify the cut sites
C.) A scientist is troubleshooting the synthesis of a cDNA library. The scientist performs both a Northern and a Southern blot. The Northern blot demonstrated the presence of RNA while the Southern blot indicated that no cDNA was present in the sample. What is likely to be the cause of the failed synthesis of the cDNA library?
(a.) proper primers (b.) temperature too cold for annealing (c.) defective reverse transcriptase (d.) too many dNTPs
