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Explain why the retention time of the parabens (parahydroxy benzoic acid esters) increases with alkyl chain length in reverse phase partition chromatography.

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Related questions

An unretained solute passes through a chromatography column in 2.6 min while the analyte requires 9.8 min.

a. Calculate the adjusted retention time (min).

b. Calculate the capacity factor (retention factor) for the analyte.

c.The volume of the mobile phase is 1.4 times the volume of the stationary phase. Find the partition coefficient for the analyte.

An open tubular chromatography column is 29.7 m long with an inner diameter of 0.552 mm. The inside wall is coated with a layer of 3.30-μm thick stationary phase. Unretained solute passes through the column in 3.89 min (tm), while a certain solute has a retention time of 16.62 min (tr).

A. What is the linear flow rate?

B. What is the volume flow rate?

C. Calculate the retention factor, k, for the solute.

D. Calculate the partition coefficient, K.

E. What fraction of time did the solute spend in the stationary phase?

A solvent passes through a chromatography column in 2.5 minutes, but the solute requires 7.7 minutes.

(a) What is the retention factor, k?

(b) What fraction of the time does the solute spend in the mobile phase in the column?

The volume of the stationary phase is 0.14 times the volume of the mobile phase in the column. (Vs = 0.14Vm.)

(c) What is the partition coefficient, K, for this system?

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