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11 Dec 2019
An open tubular chromatography column is 29.7 m long with an inner diameter of 0.552 mm. The inside wall is coated with a layer of 3.30-μm thick stationary phase. Unretained solute passes through the column in 3.89 min (tm), while a certain solute has a retention time of 16.62 min (tr).
A. What is the linear flow rate?
B. What is the volume flow rate?
C. Calculate the retention factor, k, for the solute.
D. Calculate the partition coefficient, K.
E. What fraction of time did the solute spend in the stationary phase?
An open tubular chromatography column is 29.7 m long with an inner diameter of 0.552 mm. The inside wall is coated with a layer of 3.30-μm thick stationary phase. Unretained solute passes through the column in 3.89 min (tm), while a certain solute has a retention time of 16.62 min (tr).
A. What is the linear flow rate?
B. What is the volume flow rate?
C. Calculate the retention factor, k, for the solute.
D. Calculate the partition coefficient, K.
E. What fraction of time did the solute spend in the stationary phase?
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