An electrospray quadrupole mass spectrometer was used to determine the molecular weight of a protein. The mass spectrum contains a series of clusters of peaks, where each cluster corresponds to the neutral protein (M) complexed with a different number of protons (MHnn ). Each cluster contains a series of peaks differing in mass by 1 Da which are due to the isotopic distribution of the protonated protein ion. One cluster has peaks at m/z 1515.76, 1516.06, 1516.41, 1516.71, 1517.05, 1517.36, 1517.69, 1518.00, 1518.30, and 1518.63. Find the charge (z) of the ions in this cluster from the observed separation in the m/z values of the peaks. Round the value you calculate for the charge to the nearest whole number.
b) Determine the molecular mass (M) of the neutral protein from the peak at m/z 1517.05.
An electrospray quadrupole mass spectrometer was used to determine the molecular weight of a protein. The mass spectrum contains a series of clusters of peaks, where each cluster corresponds to the neutral protein (M) complexed with a different number of protons (MHnn ). Each cluster contains a series of peaks differing in mass by 1 Da which are due to the isotopic distribution of the protonated protein ion. One cluster has peaks at m/z 1515.76, 1516.06, 1516.41, 1516.71, 1517.05, 1517.36, 1517.69, 1518.00, 1518.30, and 1518.63. Find the charge (z) of the ions in this cluster from the observed separation in the m/z values of the peaks. Round the value you calculate for the charge to the nearest whole number.
b) Determine the molecular mass (M) of the neutral protein from the peak at m/z 1517.05.
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