Please help. Labeled spectra + the questions in lab. The mol weights I calculated we're 612.64, 238.25, and 379.46 respectively
Pad 1:15 PM the nearest 0.1 amu. Peptide Molecular Formula Molecular Weight amu Glutathione (oxidized form) H NO. Gly-Tyr H, NO Val-Tyr-Val C19H20N10, 2. Students will meet at the lab location as directed by the TA/Instructor. The LCMS instrument and peptide sample will have been prepared in advance by the TA. The peptide sample will be injected and the chromatogram will be obtained. The LC chromatograms for the mass spectrometer (total ion chromatogram or TIC) will be posted on Blackboard after lab. The mass spectra for each of the compound peaks will also be posted. 4. From the masses of the molecular ion peaks in the mass spectra identify which of the LC peaks correspond to which of the peptides in the mixture. Also, identify each ion peak in the mass spectrum as either a MH, MH or M,H ion based on the m/z ratio. In addition, calculate the anticipated relative intensity of the (M+1)H peak based on isotopic abundances using equation 21-2 from the textbook, and compare it with the actual (M+1)H peak in the mass spectra for each polypeptide.
