The resolution calculated for the TLC separation of a two-component mixture is determined to be equal to 2.0. Does this represent good separation or not? Explain.

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Trinidad TremblayLv2

13 Dec 2019

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An unknown mixture was analyzed using TLC on a silica gel plate and developed in 1:6 ethyl acetate:hexane, and gave 2 spots with Rf values of 0.6 and 0.5 respectively. 1)Would this be considered a good separation between the two? Briefly explain. 2)Which spot represents the more polar compound(s)? 3)Can you say for sure how many compounds are contained in the mixture? If so, how many are there? Briefly explain. 4)If not, what further conditions might be performed to determine the number of components in the mixture or to obtain a better separation? Explain. 5)Suppose you carry out a TLC separation of acetaminophen and aspirin on silica gel using a nonpolar eluting solvent, hexane. Which should have the larger Rf value? Briefly explain. 6)What are the methods for "visualizing" spots on TLC plates that we will use in this lab?

what is thin layer chromatography (TLC) least useful for from the list below?

a. to establish that two compounds are identical

b. to determine the number of components in a mixture

c. to determine the appropriate solvent for column-chromatographic separation.

d. to determine if crystallization or distillation ma by more effective for a separation.

e. to monitor a column chromatographic separation

A mixture of two compounds eluted on silica gel TLC gave only one spot with an Rf value of 0.95. The solvent used was acetone. What, if anything, should be done differently?

a. Use a more polar solvent to improve separation

b. Use a less polar solvent to improve separation

c. The compounds are inseparable by TLC due to their polarity