ADVANCED MICROBIOLOGY Study Guide - Methyl Red, Ph Indicator, Gut Flora

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21 Jan 2023
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Discuss the principles of using these tests/culture media to determine the metabolic pathways employed by bacteria: methyl red and vogues - proskauer. In 1915, clark and lubs developed the methyl red test to differentiate between coli-aerogenes groups of enteric bacteria and followed by developing the mr-vp broth to perform two tests using the same medium. The mrvp test is used to detect the fermentation pathway used by bacteria to utilize glucose. These pathways can either be mixed acid fermentation pathway and 2,3 butanediol fermentation pathway. Methyl red test result (a) positive (red) (b) negative (yellow) The methyl red (mr) test is utilized to detect the presence of abundant amounts of acid in the fermentation process of glucose and for the maintenance of ph conditions of old cultures. Bacteria can use glucose and convert it into several end-products including lactic acid, acetic acid, or formic acid.

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