BIOTECHNOLOGY Lecture Notes - Lecture 1: Transcription Bubble, Rho Factor, Consensus Sequence
20 Dec 2022
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Document Summary
Eukaryotic transcription (alongwith role of all the transcription factors involved) Prokaryotic transcription: rna is synthesized by a single rna polymerase enzyme which contains multiple polypeptide subunits, in e. coli, the rna polymerase has subunits: two , one , one. " and one and subunit ( 2 " ). This complete enzyme is called as the holoenzyme: initiation. The first step in transcription is initiation, when the rna polymerase binds to the dna upstream (5 ) of the gene at a. 13bp around the start site to form a transcription bubble. The transition from the closed to the open complex involves structural changes in the enzymes and the opening of the dna. Double helix to reveal the template and non template strands. rna polymerase can initiate a new rna chain on a dna template and thus not need rna primer. In initiation process, polymerase-promoter complex is called the initial transcribing complex.
