MEDICAL TECHNOLOGY Lecture Notes - Lecture 1: Immunodiffusion, Precipitin, Nephelometer
28 Oct 2022
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Reaction between a soluble antigen + soluble antibody. Rbcs) aggregate to form larger complexes in presence of a specific antibody. Ag and ab: once ag and ab binds together, this is force that keeps them bonded. Types of precipitation: precipitation in a fluid medium. Adding of reactants to support medium and let it migrate or diffuse by their own means. Carry in a support medium (agar, gel), but no electricity: linear- positive result: presence of precipitin line or a purple band, radial - positive result: radius/ring, one-dimensional - one reactant use, ex. Only ab moves to the ag reagent: two-dimensional - two reactant use, both ab and ag move, precipitation by electrophoretic techniques. Reactants move and meet because of the application of electricity. Passive immunodiffusion support medium: used to read/determine precipitation results (ex. Reactants: ag and ab added to the gel: principle involved: diffusion.
