BIO 3124 Lecture Notes - Lecture 19: Rna-Seq, Serostatus, Mycoplasma
12 Dec 2016
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Document Summary
Establish and confirm the etiology of the disease. Track the progression of the infection in the patient. Biochemical tests - make a biochemical fingerprint of organism. Oxygen requirements: carbon sources used, oxidative or fermentative metabolism, metabolic byproducts. When antibodies react with multiple epitopes on soluble antigens, there is formation of networks which generate an insoluble precipitate: aggregates become insoluble and form a precipitate. Precipitation reactions can take place in solution or in gels such as agar. The precipitate formation is influenced by the concentration of ag - ab. Add a limiting concentration of complement proteins (c1-c9) Impt to add limiting amount of complement proteins: will start classical cascade - so complement fixation. Complement proteins will be completely used up: complement proteins will not be used if no complex formed. Add sensitized rbc: have reacted with igg or igm. Test that determines whether complement was used as a result of specific binding of ab to ag.
