BIOLOGY Lecture Notes - Lecture 6: Biomolecule, Glycogen, Reagent
Document Summary
Give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. Keeping the sample refrigerated or frozen prior to glycogen isolation. Biomolecule, also called biological molecule, is a molecule that is present in organisms which are necessary to one or more biological processes, such as cell division, morphogenesis, or development. Biomolecules include macromolecules or polyanions, such as proteins, carbohydrates, It also includes micro molecules such as primary metabolites, secondary metabolites lipids and nucleic acids. and natural products. The methods involving the isolation of biomolecules include centrifugation, filtration, absorption and chromatography. Role of reagent in the isolation or analysis of glycogen: The glycogen isolation is done by using the reagents such as potassium hydroxide, ethanol, and trichloroacetic acid (tca) for alkaline hydrolysis. Glycogen isolation involves some human disorders that affect the metabolism of glycogen in the body. Glycogen structure consists of -1,4 glycosidic bonds in its main chain. When compared to amylopectin it has - 1,6 glycosidic bonds.
