MIC281 Lecture 3: Final: REGULATIONS INSIDE THE LABORATORY (experiment no 1)

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16 Aug 2022

School

Amrita Vishwa Vidyapeetham - Amritapuri Campus

Department

Health Science

Course

MIC281

Professor

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Document Summary

Enter the laboratory with a clean coat to protect your clothes. Sponge off the top of your lab desk with disinfectants like phenol both before and after the experiment. Place all the waste materials in the waste can and used glassware on the tray. Many microscopic organisms any be pathogenic, therefore avoid any hand to mouth operations. Food and drinks must not be taken in lab. Whenever a living culture is spilled on the desk or floor at once disinfect the place. Take proper care of microscope and other lab equipment. Handle all cultures carefully according to the instructions. In case of any accident report immediately and take precaution to avoid any accidents. Before starting an experiment you should know you are going to do and take note of all the observations. Never lay or hang lab coat on the lab desk. Do not have valuables in the lab coat.

Related Questions

Pre-Laboratory Questions

1. Why do bacterial cultures need to be incubated, usually for at least 24 hours, before we can see evidence of bacterial growth that can be observed with the unaided eye?

2. In what ways are the media used in this investigation selective?

3. In what ways are the media used in this investigation differential?

4. Consider a mixture of bacterial species from the same genus, such as Staphylococcus. Some members of this genus are harmful pathogens, while others are not. Would a selective medium alone be a good tool to separate the harmful species from the non-harmful species? Explain your answer.

Post-Lab Questions

1. Would simply adding a pH indicator like phenol red to a general-purpose medium, such as nutrient agar, result in a medium that is selective, differential, both, or neither? Why or why not?

2. Why were you instructed to inoculate each plate with three separate streaks, one of each bacterial species, instead of combining all three species and streaking them all onto the plate together?

3. Blood agar and chocolate agar are commonly-used media in many clinical and research laboratories. Why do you think they were not included in your kit?

4. Were there any discrepancies between what you saw in your experiment and what you expected to see based on the lab manual and your readings in this section?

A) In this hypothetical scenario, your instructor asks you to determine the number of organisms in a water sample. Observation of your dilution plates reveals the presence of spreading colonial forms on some of the culture plates. What is the rationale for the elimination of these plate counts from your experimental data.

B) A patient exhibits a boil on his neck. You, as a microbiology technician, are asked to identify the causative organism and determine whether it is pathogenic. Describe the procedure that you would follow to make this determination.

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QUESTION 1: Escherichia coli is characterized as a facultative anaerobe. You set up an experiment to examine the growth of Escherichia coli in three environments: a GasPak jar (0% oxygen), a candle jar (10% oxygen), and the benchtop (22% oxygen). Aside from differing oxygen levels, all other culture conditions (such as temperature and media used) were kept constant. Which of the following represents the most likely outcome of the experiment?

A. E. coli will have heavy growth on the benchtop, moderate growth in the candle jar, and no growth in the GasPak jar.

B. E. coli will have heavy growth on the benchtop but moderate growth in the candle jar and the GasPak jar.

C. E. coli will have heavy growth in the GasPak jar and moderate growth in the candle jar and the benchtop.

D. E. coli should grow equally in all three growth environments.

QUESTION 2: Chloroflexus aurantiacus is a photoautotrophic bacterium that grows in hot springs. Which of the following serves as an energy source for C. aurantiacus?

A. The sun

B. Glucose (C₆H₁₂O₆)

C. C. aurantiacus does not need an energy source

D. Carbon dioxide (CO₂)

QUESTION 3: You are a manufacturer of polypropylene hypodermic syringes. Which of the following methods would be best for ensuring sterility of this heat-sensitive material?

A. Non-ionizing radiation

B. Ionizing radiation

C. Pasteurization

D. Autoclave

QUESTION 4: What is the primary difference between a disinfectant and an antiseptic?

A. Disinfectants are low-level germicides, whereas antiseptics are high-level germicides.

B. A disinfectant is microbiocidal, whereas an antiseptic is microbiostatic.

C. A disinfectant is used to decontaminate critical equipment, whereas an antiseptic is used to decontaminate semicritical equipment.

D. A disinfectant is used to decontaminate lifeless objects, whereas an antiseptic is applied to living tissue.

QUESTION 5: Your patient is receiving a spinal fusion to correct a skeletal defect. Which of the following germicides is recommended to sterilize the bone used for the transplantation?

MIC281 Lecture 3: Final: REGULATIONS INSIDE THE LABORATORY (experiment no 1)

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