HSC200H5 Study Guide - Quiz Guide: Color Theory, Color Temperature

28. There are two alleles (Al and A2) at the locus for flower colour. If 36% of the plants in the population are AJA1 for flower colour, what is the frequency of plants with the heterozygous genotype? a. 0.16 b. 0.36 c. 0.24 d. 0.48

Why is such a seemingly complicated scheme used for LacZ complementation (X-gal colour selection and α-peptide complementation), in which most of the lacZ gene is in the host chromosome and the small α-peptide resides in the cloning vector? In other words, why not have the entire lacZ gene in the vector? Give two reasons. [3 marks] [Clue: From the relevant lectures and the Lab Manual, based on colour selection (Lecture 4) and the sizes of recombinants (Lecture 5)]