Biology And Biomedical Sciences BIOL 2960 Lecture Notes - Lecture 29: Dna-Binding Domain, Telomere, Xist (Gene)
14 Aug 2018
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Document Summary
In eukaryotes transcription and processing of the mrna takes place in the nucleus and translation happens in the cytoplasm: also has a time separation, prokaryotes really don"t have processing, but trans. Transcript processing: extensive processing of primary transcript destined to become mrna in eukaryotes. General transcription factors cis-acting elements: dna regulatory sequences that bind proteins: are more varied and can be positioned in different configurations relative to the coding sequence, an example: operator dna. For bacteria: ground state of transcription: ground state is always on, template for transcription is protein free, activators enhance weak polymerase binding, repressors interfere with polymerase binding, rna polymerase binds to dna, promoter composition: promoters are dna. Intron: part that is spliced out and not actually made into mrna: exon: part that is stitched together and coded into mrna. In a test tube (i. e. naked dna): core promoter: transcription factors & rna polymerase to non-chromatin.
