BIO 3110 Lecture Notes - Lecture 1: Mycolic Acid, Proteus Vulgaris, Blood Gas Tension
Document Summary
Ocular lens- rest within the upper end of binocular tubes (cid:0) always 10x. Iris diaphragm- controls the amount of light reaching the specimen. Coarse adjustment- will allow you to get an approximate focus on the specimen. Fine adjustment- produces the final focus on the specimen. Stage traverse- secures the slide with a sprint action clip . Newer microscopes in the laboratory are parfocal, meaning that once an object has been placed into focus with one objective lens, it remains in focus when changing to another objective lens with only fine focus adjustment required. Phase contrast- used for wet mount, observing live organisms. True motility vs brownian motility- brownian motility is when vibration of organisms produced by the bombardment of water molecules and solute molecules. Convection motion- flow of water currents in the wet mounts as areas of the slide heat up from warmth of the light source cause motion. Neither brownian motion nor convection is true motility.
