BIOL 2604 Lecture Notes - Lecture 1: Minimum Inhibitory Concentration, Formaldehyde, Growth Factor
Document Summary
Bacteriolytic agents: blows em up, target those starch alpha 1,4 bonds between (g) and (m), lysed! Make serial dilutions of the antimicrobial find lowest concentration/most dilute that totally inhibits growth. Paper disks containing antimicrobial chemical are placed on plates that have been inoculated to grow on a lawn, totally confluent. Diffusion of chemical into medium makes a gradient about disc. Sensitivity is determined by diameter of zone of growth inhibition around the disc. Size of zone of inhibition can be related for usefulness in treatment. *decimal reduction: reduction of population by a factor of 10. Gaseous infusion of chemicals like formaldehyde, ethelene oxide. Kill most organisms (not endospores, really resistant stuff) Sulfonamides stop folic acid synthesis by analogging paba [para amino benzoic acid] usually and that is needed for nucleic acid synthesis: isoniazid [mess with mycobacteria, have waxy stuff on em, nucleic acid analogs. Quinolones: dna gyrase inhibitors stop pulling of dna strands apart for replication, e. g. , nalidixic acid, ciprofloxin.
