BIOL 2604 Study Guide - Quiz Guide: Chicken Anaemia Virus, Baltimore Classification, Plaque-Forming Unit
Document Summary
Heat is the most widely used method for sterilization (killing of all organisms) Sterilization must eliminate all heat resistant organisms, endospores. An autoclave permits the application of steamed heat above the boiling point of water under pressure. Pasteurization does not sterilize liquids, but reduces microbial load, killing most microorganisms and spoilage (milk gets pasteurized to be drinkable: less damaging to food products than heat sterilization. Radiation sterilization: 220-300nm wavelength, causes dna damage and surface decontamination. Ionizing radiation- damage though generation of highly reactive ions, absorbed radiation dose measured in rads or grays (lethal dose to kill humans is 10 grays) Depth filters: fibrous nature, used to pre-filter liquids, sterilization of air. Standard membrane filter: 80% open pore, traps filtrate on surface, common heat sensitive liquid sterilization filter. Nucleopore membrane filter: etching polycarbonate film after nuclear radiation. Bacteriolytic: blows em up, target starch alpha 1,4 bonds between g and m, lysed!
