BIOL 2604 Lecture Notes - Lecture 2: Bacterial Gliding, Endospore, Flagellum
Document Summary
Dark field for flagella [phase contrast helps too] Endospores *made by bacillus and clostridium - and barely others* Withstand extremes heat, chemical treatment, desiccation [dried out], hydration, and radiation. Form during times of no food/bad growth conditions allow for survival + dispersion. Can remain *dormant* for (possibly) thousands of years. Germinate when conditions are favorable giving rise to normal, vegetative forms of the bacterium. May even have an exosporium, even more holding. Has a coating on it called the spore coat. Calcium-dicolinic acid reduces water availability and intercalates in dna. Sasp*s bind strongly to dna [small acid soluble proteins] convert the dna into a super dna that is more compact and uv-resistant. Vegetative cell sporulating cell [endospore is growing inside. Mother cell creates, has a spore inside of itself] Mature spore lyses away from main cell. Keeps splitting in half, completes the septum with formation of distinct walls. Dna gets dense after replication at vegetative cell.
