BIOL 1105 Lecture Notes - Lecture 19: Haemophilus Influenzae, Genomics, Chromosome
14 Nov 2017
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Document Summary
Haemophilus influenza, a bacterial genome was sequenced in the mid 1990s. Genetic maps provide relative locations of genes: determined by recombination frequency. First knowledge about genomes came from constructing genetic maps. Physical maps show us the actual location of landmarks: Restriction maps are a common type of physical map: dna is cut with restriction enzymes and the banding pattern is analyzed to generate a map, distances on this type of map can be measured in base pairs (kilobases) Chromosome banding and fluorescence in situ hybridization (fish) Fish-dna probes with fluorescent labels hybridize at the site of a specific gene on a chromosome. Sequence-tagged sites are small stretches of dna that are unique to the genome of a species and only occur once. Pcr can be used to detect the presence of a particular sts. Fragments of dna can be pieced together by analyzing overlapping sts sites.
