INTER-LS 101 Lecture Notes - Lecture 3: Methyl Group, Uracil, Thymine
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Which of the following did Erwin Chargaff observe?
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The melting temperature (Tm) of a DNA duplex is:
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Given your knowledge and the descriptions of each of thesemethods, which of the following does NOT rely on the ability ofnucleic acids to hybridize with each other?
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Which of the following is a possible reason why DNA uses thymineinstead of uracil?
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Which of the following is an enzyme used in cloning to breakcovalent bonds?
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Which of the following could you use to remove the 5' phosphatesafter cleavage of a plasmid with a single type of restrictionenzyme to ensure that the plasmid would not simply fuse backtogether upon addition of ligase?
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Which of the following would you use to create a cDNA librarythat you would not use for a genomic library?
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A plasmid vector typically has which of the followingfeatures?
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Which of the following is FALSE of genomic or cDNAlibraries?
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Which of the following is NOT a typical component of apolymerase chain reaction (PCR)?
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How can a fusion protein be formed?
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Why is green fluorescent protein (GFP) so useful in visualizingfusion proteins in eukaryotes?
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You wish to determine the cellular location of a protein butunfortunately it is not particularly antigenic (can t develop astrong antibody response). How could you solve this problem?
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A positive result for the yeast two-hybrid analysis means thefollowing:
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Which of the following is the approximate size of the humangenome?
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Which of the following is correct about the structure orlocation of genes?
Question 22 options:
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If one is testing a goodness of fit to a 9:3:3:1 ratio, how many degrees of freedom would be associated with the Chi-square?
1
2
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4
not enough information to determine
Which of the following choices does not describe a type of posttranslational modification of proteins?
The amino terminal methionine may be removed. |
Glycoproteins are produced by the attachment of phosphates to newly synthesized proteins. |
Precursor proteins are cleaved and trimmed by protease enzymes to produce a functional protein. |
Molecular chaperones help proteins fold correctly. |
Amino acids in the amino terminal of the protein can be acetylated. |
A eukaryotic DNA sequence that affects transcription at distant promoters is called a(n)
long distance promotor |
regulator |
operon |
enhancer |
DNA methylation may be a significant mode of genetic regulation in eukaryotes. Meythylation refers to
altering RNA polymerase activity by adding methyl-groups |
changes in DNA-DNA hydrogen bonding |
altering translation activity via methylated tRNAs |
adding methyl groups to certain cytosines in DNA |
If a nucleotide lacks a 3â-OH group, what will happen?
itâs transformed into RNA |
another nucleotide can bond at the 3â site during replication |
replication will halt |
no effect |
Which of the following describe posttranscriptional modifications commonly seen in mRNA of eukaryotes?
5â capping, 3â-poly-A tail addition, splicing |
3â capping, 5â poly-A tail addition, splicing |
heteroduplex formation |
5â capping, 3âpolyA tail addition, removal of exons |
In birds, females are the heterogametic sex. This means that
females have chromosomes in pairs. |
emales produce eggs. |
females determine the gender of their offspring. |
males have two identical sex chromosomes (ZZ) An anticodon on a tRNA has the sequence 5'âGCAâ3'. What would be the effect on translation if the G in the anticodon were mutated to a U?
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