CHEM 451 Lecture Notes - Lecture 16: Atp Hydrolysis, Intrinsically Disordered Proteins, Hsp70
Document Summary
3 major domains = nucleotide binding domain with adp bound, flexible linker to substrate binding domain (beta sandwich), helical lid region. Yellow squiggle is the substrate that binds to substrate binding domain. Lid clamps over substrate to keep it in position - adp bound form of structure. In fig b atp binds, lid region opens up and is docked against nucleotide binding domain. To open lid, atp needs to bind in order for lid to be open (binding causes conformational change -> lid docking against nucleotide binding domain) When lid is open, atp + hsp70 is bound. When atp binds then the lid opens up and exposes the substrate binding domain to the substrate/ ready to bind. In general, it binds to misfolded part of protein and provides environment to refold correctly. Implication = high k on /k off when atp is bound and lid is open - substrate hasn"t bound yet.
