MCDB 423 Lecture Notes - Lecture 17: Lamellipodium, Cell Membrane, Cytochalasin

• School: U of M
• Department: Molecular, Cellular and Developmental Biology
• Course: MCDB 423
• Professor: John Kuwada

Document Summary

Once the flipodia touches the substrate, the whole growth cone gets pulled behind that site of contact (left movie) The actual contact it makes with the bead is very transient. The semaphorine in this case is fused to the beads (but normally it can diffuse through the envrionment) slide 3. You can see that the growth cone is very dynamic without any cues but addition of semaphorin collapses it (left) A growth cone turning assay generates an artifical gradient by using a micro spitter (right) A s(cid:373)all pieppet releases s(cid:373)all pulses of the (cid:272)ue (cid:894)i(cid:374) this (cid:272)ase it"s (cid:374)etri(cid:374)(cid:895) Small pulses of netrin are released and because they rapidly diffuse through, if you pulse it you can have a higher concentration of netrin at the source slide 4. The paper quantifies the growth turning through this assay. The pieppet creates a gradient around the source. It"s applied to a growi(cid:374)g (cid:272)ulture for 30 (cid:373)i(cid:374)s.