BIOLCHEM 415 Lecture 32: Lecture32_Chapter35_Apr1_Vojtek_2016

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Dna recombination in repair in biological processes in genetic engineering. Substitutions incorporation of an incorrect base during replication that escapes proofreading by dna polymerases: transitions: Pyrimidine to pyrimidine (e. g. c to t: transversions: purine to pyrimidine (a to c, a to t, g to c, or g to t) Pyrimidine to purine (c to a, t to a, c to g, or t to g) Breaks in one or both dna strands, which may halt dna synthesis altogether. translesion polymerases: replicate across the damage & generate a rough draft of the damaged sequence. Bases can be damaged by mutagens mutagens: chemicals that alter speci c bases after replication is complete. Reactive oxygen species, such as hydroxyl radicals, can damage dna hydroxyl radicals. H: 8-oxoguanine base pairs with adenine instead of cytosine, as a result, c is mutated to an a = a transversion mutation. A pairs with t but hypoxanthine base pairs with c.

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