BIO 152 Lecture Notes - Lecture 3: Glycerol, Extracellular Fluid, Enzyme Inhibitor

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Everything that comes before the enzyme would build up. Anything after that enzyme would not be produced. Enzyme products regulate enzymes: negative feedback. High concentration of products can bind to allosteric sites to block function of enzyme that starts metabolic pathway. When you make enough of something or get to a level, the mechanism that makes the substance is turned off: low product (active) Substrate does bind to the active site and produces intermediates that binds to all other enzymes needed to produce the product: high product (inactive) Substrate does bind to the active site and automatically produces product without producing the intermediates. Allosteric binding results in feedback inhibition; enzyme 1 cannot bind to substrate. Transcriptional regulation: promoters, make enzyme or not, long term way to regulate enzymes. Phosphorylation of enzymes: turn on/off, faster, reversible, adding a phosphate group to an enzyme, and changes the shape of the protein. This changes the function of the protein.

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