CBIO 3400 Lecture Notes - Lecture 6: Rna Polymerase Ii, Tata Box, Tata-Binding Protein
7 Apr 2019
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Document Summary
Transcription: the 3 main steps of eukaryotic transcription: initiation- transcription factors adhere to the tata box in the promoter signaling rna polymerase ii to attach. Additional transcription factors attach and the transcription initiation complex is formed. Promoters: promoters are upstream, utrs (untranslated regions) Transcription factors: transcription factors- they can read the dna to determine if they should bind to and activate transcription from a particular gene, each element below has a consensus sequence associated with it. Inr: initiator element: dpe: downstream promoter element, tfiia binds and stabilizes the tfiid complex, tfiib binds to bre. Major component that recruits the rna to the locus we are going to transcribe. Also determines the start site of transcription: main thing that does the work. Tfiif is made up of 2 subunits (rap38 and rap74. Starts to recruit tfiie which is important f or bringing in tfiih to the complex by priming the initiation complex for promoter clearance.
