CMMB 411 Lecture Notes - Lecture 29: Rna Polymerase Ii, Tata Box, Tata-Binding Protein

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rosefrog223

18 Dec 2018

School

University of Calgary

Department

Cellular, Molecular and Microbial Biology

Course

CMMB 411

Professor

Sui-Lam Wong

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Document Summary

Eukaryotes have 3 rna polymerases, whereas prokaryotes only have 1. Rna polymerase in all organisms contains 2 alpha, 2 beta, and 1 omega subunits. Eukaryotic polymerase i, ii, and iii have 7 to 11 extra subunits which are mainly specific to each polymerase. Rna polymerases don"t share the same subunits, they are separate genes that are specialized for the transcription of each polymerase. Eukaryotic polymerases are analogous to archaeal protein. Core promoter for rna polymerase ii (pol ii) Promoters usually contain a subset of the possible core promoter elements. Promoters are constant in bacteria, in eukaryotes there are a lot of elements with substantial variability. Need to have enough accuracy of consensus sequences to have proper binding. Proteins that recognize the specific dna sequences. Tf2b (tfiib): in humans, this is a transcription factor1 for polymerase ii. Tbp: tata binding protein, this will recognize the tata box. Core promoter: elements identified by basic machinery and rna polymerase.

Related Questions

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

CMMB 411 Lecture Notes - Lecture 29: Rna Polymerase Ii, Tata Box, Tata-Binding Protein

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