CHEM 6A Lecture 21: Week 10 L2 (Dec 6th 2018)

126 views2 pages

Verified Note

coralkangaroo200

8 Dec 2018

School

UCSD

Department

Chemistry and Biochemistry

Course

CHEM 6A

Professor

Kim Albizati

For unlimited access to Class Notes, a Class+ subscription is required.

CHEM 6A verified notes

21/21View all

CHEM 6A Lecture 2: Week 1 L1

CHEM 6A Lecture 3: Week 1 L2

CHEM 6A Lecture 21: Week 10 L2 (Dec 6th 2018)

Related Questions

- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 1.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 2.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 3.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 4.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 5.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 6.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 7.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 8.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 9.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 10.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 11.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 12.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 13.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 14.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 15.
- A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P.	Step 16.

A.	Don appropriate personal protective equipment (PPE).
B.	Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10^-5 cumulative dilution.
C.	Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10^-7 cumulative dilution.
D.	Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10^-4.
E.	Allow the inocula to soak into the plates before proceeding.
F.	Aseptically transfer 0.1 mL from dilution tube 2 to plate A₁. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
G.	Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10^-6 cumulative dilution.
H.	Obtain eight plates, organize them int four pairs and label them A₁, A₂, B₁, B₂, etc.
I.	Keep accurate records in your lab notebook. Clean up the lab benches, doff PPE, and wash hands.
J.	Repeate the procedure with plate A₂ and label both plates "10^-5mL of original sample," or simply "10^-5" Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10^-4 dilution, but the plate is a 10^-5 dilution!!
K.	Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
L.	Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
M.	Incubate the plates at 35â for 24 to 48 hours.
N.	Obtain five dilution tubes and label them 1â5, respectively. Make sure they remain covered until needed.
O.	Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
P.	Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10^-2 dilution

1. _____ Alpha helix

2. _____ Disulfide bonds

3. _____ Okazaki fragments

4. _____ Hydrophobic interactions

5. _____ Complementary pair having 3 hydrogen bonds

6. _____ Globular protein thar carries 4 heme units

7. _____ Insoluable, used as structural components

8. _____ Cofactor

9. _____ Side chain interactions

10. _____ Sequence of monomers

11. _____ Delivers amino acids to the ribosomes

12. _____ Thymine, cytocine, and uracil

13. _____ Beta plated sheet

14. _____ Complementary pair having only 2 hydrogenbonds

15. _____ Continuous replication

16. _____ Contains both introns and exons

17. _____ Adenine and guanince

18. _____ Process that removes introns

19. _____ DNA to hmRNA

20. _____ Process of protein construction

21. _____ f-MET removal, chaperone proteins, etc.

22. _____ Contains codons

A. Mrna

B. Interchain hydrogen bonding

C. Intrachain hydrogen bonding

D. Hemoglobin

E. Primary structure

F. Methionine

G. Tertiary structure

H. tRNA

J. hnRNA

K. Post transcription processing

L. Translation

M. Post translation processing

N. Transcription

O. Guanine - Cytosine pair

P. Adenine - Thymine pair

Q. Purine derivative bases

R. Pyrimidine derivative bases

S. Fibrous protein

T. Conjugated enzyme

U. Lagging strand

V. Leading Strand

W. Carbonic anhydrase

cyansnail129

1. _____ Alpha helix

2. _____ Disulfide bonds

3. _____ Okazaki fragments

4. _____ Hydrophobic interactions

5. _____ Complementary pair having 3 hydrogen bonds

6. _____ Globular protein thar carries 4 heme units

7. _____ Insoluable, used as structural components

8. _____ Cofactor

9. _____ Side chain interactions

10. _____ Sequence of monomers

11. _____ Delivers amino acids to the ribosomes

12. _____ Thymine, cytocine, and uracil

13. _____ Beta plated sheet

14. _____ Complementary pair having only 2 hydrogenbonds

15. _____ Continuous replication

16. _____ Contains both introns and exons

17. _____ Adenine and guanince

18. _____ Process that removes introns

19. _____ DNA to hmRNA

20. _____ Process of protein construction

21. _____ f-MET removal, chaperone proteins, etc.

22. _____ Contains codons

A. Mrna

B. Interchain hydrogen bonding

C. Intrachain hydrogen bonding

D. Hemoglobin

E. Primary structure

F. Methionine

G. Tertiary structure

H. tRNA

J. hnRNA

K. Post transcription processing

L. Translation

M. Post translation processing

N. Transcription

O. Guanine - Cytosine pair

P. Adenine - Thymine pair

Q. Purine derivative bases

R. Pyrimidine derivative bases

S. Fibrous protein

T. Conjugated enzyme

U. Lagging strand

V. Leading Strand

W. Carbonic anhydrase

CHEM 6A Lecture 21: Week 10 L2 (Dec 6th 2018)

CHEM 6A Lecture 2: Week 1 L1

CHEM 6A Lecture 3: Week 1 L2

CHEM 6A Lecture 21: Week 10 L2 (Dec 6th 2018)

Get access

Related textbook solutions

Chemistry: Structure and Properties

Basic Chemistry

Principles of Chemistry Molecular Approach

Chemistry: Structure and Properties

Principles of Chemistry Molecular Approach

Chemistry: A Molecular Approach

Chemistry: A Molecular Approach

Principles of Chemistry: A Molecular Approach

Chemistry: The Central Science

Related Documents

CHEM 6A Final: CHEM 6A Final Exam 2011

CHEM 6A Final: CHEM 6A Final Exam 2011

CHEM 6A Study Guide - Final Guide: Gas Constant, Avogadro Constant, Scantron Corporation

Related Questions