BIO SCI D103 Lecture Notes - Lecture 11: Heat Shock Protein, Conserved Sequence, Gel Electrophoresis
18 Mar 2014
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Writing questions: what if we mutate this protein, new knowledge nugget a. Gel electrophoresis: clicker, mutation in ndna leads to a complete block in mito protein import i. Which protein could be affected by this mutation. None of these conditions above prevent mito protein. Protease will not be able to digest imported proteins. More similar between if classes a. a. b. Purified protein with signaling signal a: wt mitochondria, mix and observe protein import. Add protease to mixture i: mechanistic insight b. c. Initial protein is larger than imported protein: sorting signals - targets proteins, mitochondrial matrix i. Recognized by specific mito receptors: mitochondrial proteins are mostly imported, hsp70. Use atp to keep it in an unfolded state: mitochondrial protein import b. c. close together d. Import receptor is located next to the import channel. Tom and tidownm are found where the outer and inner membrane are. Has a pulling protein that provides directionality to the process i. ii.
