BIO SCI 97 Lecture Notes - Lecture 1: Tumor Suppressor Gene, Spermatocyte, P53
1 Sep 2016
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Bio 97 lesson learning objectives, fall 2015, prof. lee bardwell, univ. of. Lecture 19: from gene to protein to disease. Sickle cell disease: caused by abnormality of hemoglobin- oxygen carrying protein in. Rbc. shortens life span of rbc from 120 days to 10-20 days. Gives victim anemia and low red blood cells. Damages and kills tissues causing muscle pain and damage to organs. runs in families, passed from gen to gen=hereditary disease. Hemoglobin: made of 2 alpha and 2 beta. Compare/contrast how gel electrophoresis works for the following. Gel electrophoresis: smaller molecules move faster, more negatives particles move faster, tight condensed sphere molecules move faster than linear. blotting- moving nucleic acids or proteins from gel to membrane. For dna gels (review of 97. 12 material) Southern=dna: 1. take dna sample and use restriction enzyme to cut that gene, run gel, bands run in gel, put filter on top of gel, paper towel on top of filter and bands stick to filter.
