BIO SCI 97 Lecture Notes - Lecture 16: Embryonic Stem Cell, Somatic Cell Nuclear Transfer, Diabetes Mellitus Type 1
29 Mar 2018
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Advantage of bacterial plasmids instead of pcr. Pcr is limited in the length of the dna it can produce. Example of the practical use of recombinant dna technology. Type 1 diabetes used to be deadly weight. This led to severe allergic reactions production. How to get recombinant dna into cells. Present: two chains were made separately and then put together in e. coli. It"s impractical to transform all the cells in a large organism. If you start from a whole organism, you can"t use a trick like killing cells. Get rid of cells where the desired recombination event. Select the ones with the presence of engineered construct and with the absence of the negative selection marker. Common negative selection marker: thymidine kinase didn"t happen. Derive animals from stem cells with engineered genome. First animal to be cloned from a differentiated cell. How to clone an animal with somatic cell nuclear transfer. Take dna from a cell of the donor animal.
