BIO 198 Lecture Notes - Lecture 24: Telomere, Telomerase, Dna Replication
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15 Oct 2016
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Cooperativity of binding: ligand that increases other ligands to bind. Ssb proteins have positive cooperativity of binding and one protein will allow more ssb proteins to bind. Sliding clamp: can slide only over double stranded dna. Unwinding of the dna strands require energy in atp and hydrolysis. Dna; you need a loader (dna c) is the helicase loader. However, e. coli chromosome is a duplex circle and we open up the circular dna circle by what replication intermediates looked like: to see which direction is the replication. Grew spores in radioactive thymine and before lysed the cells, added more radioactivity in specific activity. They could see the intermediates and new synthesized forks: The leading strand on one fork is the lagging fork on the other (it is bidirectional and starts on the center but more away from each other): Dna a is an initiator protein: gets things started and then goes away (controls when replication is supposed to start)
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