BIOL 1081 Lecture Notes - Lecture 13: Light-Dependent Reactions, Cellular Respiration, Thylakoid

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1 Jun 2018
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Ch. 8 LO
Carbon Fixation & Photorespiration Reading: Sections 8.6-8.7 in textbook
1. List the inputs and outputs of the Calvin cycle, describing both where it occurs and its purpose.
Inputs
o CO2, ATP, and NADPH, water
Outputs
o ADP, G3P, NADP+
Location
o Stroma of chloroplast
Purpose
o Set of chemical reactions that ours to overt su’s eergy ito storale for gluose;
making sugars
2. Name the enzyme that fixes CO2 in the Calvin cycle and describe the reaction that it carries out, as well as
the name and structure of the first stable intermediate in the cycle.
RuBP carboxylase fixes CO2 to RuBP
Carbon fixation reaction generate 2 molecules of the 3-carbon acid PGA (3-phosphoglycerate)
It catalyzes the entry step of CO2 into the Calvin cycle
3. Explain the cause of the conflict that leaves have between gas exchange and water loss, and describe the
adaptations that leaves typically have to minimize water loss.
Plants want to open/ close their stomata for gas exchange but this also requires water loss
When leaves open the stomata it causes CO2 to come in but H20 to be lost
When the stomata are closed it causes a buildup of O2 and RuBisCO will use O2 instead of CO2
4. Describe a major limitation of RuBisCO and how it is exacerbated by the above-mentioned adaptations
that minimize water losses in the leaf.
Atmospheric oxygen competes with CO2 as a substrate for rubisco, giving rise to
photorespiration
Has to remove bound RuBP in order to be active
During photorespiration, rubisco uses O2 as a substrate
5. Name the two groups of plants that have evolved successful solutions to photorespiration and describe
how they operate, including the key processes, CO2-fixing enzymes, and products that are involved.
C4 plants- corn, sugarcane
o Initially fix carbon using PEP carboxylase in mesophyll cells produces organic acid
oxaloacetate Malate and transported to bundle sheath cells
o Malate in bundle sheath cells is decarboxylated to produce pyruvate and CO2
o Chloroplasts are located in both the mesophyll and bundle sheath cells
CAM plants-succulent
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