BIO 201 Lecture Notes - Lecture 10: Molecular Motor, Muscle Contraction, Dynein
6 Sep 2018
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**** wed 2/22 exam 1 (lectures 1-10)***** How molecular motors were discovered the sliding assay. Stimulation from neuron ca++ influx into muscle cells contraction. Approach : purify different proteins from muscle, attach each to glass slide, add fluorescent actin (capped at both ends to prevent polymerization and depolymerization, add atp. Myosi(cid:374) causes acti(cid:374) fila(cid:373)e(cid:374)ts to (cid:862)slide(cid:863) hydro to adp. Actin is not needed to do a filament sliding assay with kinesin. Atp to the left, and adp to the right. Rigor mortis: the third stage of death, when limbs of a dead animal stiffen due to muscle contraction. Are motor proteins responsible for transporting cargo along microtubules. Kinesin and dynein microtubule sliding motor proteins. Plus end-directed mt motor (moves toward the plus end) Myosin is a plus end-directed actin motor protein (muscle contraction) Kinesin is a plus end-directed mt motor protein (anterograde transport) Dynein is a minus end-directed mt motor protein (retrograde transport)
