Biology BIO 2440 Lecture Notes - Lecture 5: Methylene Blue, Mannitol, Eosin
Document Summary
Lab 5: selective & differential media and biochemical tests. Selective media allows for the growth of desired organisms and prevents the growth of undesired. Differential media permits the growth of many different types of organisms, but allows you to differentiate them based on appearance. Macconkey"s agar, eosin methylene blue agar, blood agar, mannitol salt agar. Enrichment media contains nutrients that enhance the growth of a desired organism. General purpose media supports growth of most bacteria. Enzyme substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. Catalyst substance that increases the rate of a chemical reaction without itself undergoing any permanent chemical change. Mannitol salt agar (msa) incubate for 24 hours in a 37 c incubator. Selects for gram-positive halophiles: because high salt concentration. This is generally bacteria from the staphylococcus genus. Media starts off as red with a neutral ph of 7.