BIO 327 Lecture 8: Lecture 8
Document Summary
Break apart the cells and tissues by physical or chemical means. End up with membrane bound organelles and molecules. Differential centrifugation- repeat the centrifugation at faster and faster speeds. Larger, heavier material forms a pellet at the bottom. Column chromatography- proteins are placed in a column and immersed in solvent. They slow down at different rates based on size, charge, ability to bind to certain chemical groups, and hydrophobicity. Molecules that interact with the column will take longer to come out. Gel filtration chromatography- can separate proteins based on their size and shape. The matrix is made up of beads that can be of a certain shape. Smaller molecules that can fit inside the bead take longer to filter out. Gel electrophoresis- applies an electric field to a solution with proteins. The direction and speed that the proteins travel reflects their charge and size. Antibodies can be used to purify and detect proteins.
