BIO 361 Lecture Notes - Lecture 4: Peptide, Gene Expression, Phenylalanine
27 Jun 2016
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Biochemistry professor sanford simon stony brook summer 2016. The primary structure of a protein is the amino acid sequence of the polypeptide chain. 100 is lower end limit, because proteins cannot fold with less than 100 residues. 1000 is higher end limit, because it is difficult to produce a long mrna for over a 1000 protein residue, as it causes issues with stability and folding an abnormally long length of a protein. Some large proteins are composed of multiple subunits. Overcomes problem of creating a single long protein. The properties of a protein are affected by the primary structure. 10^7 protein molecules in an e coli cell. Overexpression can significantly increase the amount of our protein prior to purification. Gene expression and translation of our desired protein enables it to stand out amongst 10^7 protein molecules. Proteins need to be in a controlled environment. Low temperature (slow the rate of undesired reactions)
