BIO 103 Lecture Notes - Lecture 28: Somatic Cell Nuclear Transfer, Embryonic Stem Cell, Roslin Institute

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Therapeutic cloning - involves growing new tissues or organs. Reproductive cloning - creating new individuals or animals. This is different from cloning a gene, which is the process of identifying, isolating, and making copies of a gene. The process of cloning by somatic cell nuclear transfer creates an embryo. The embryo can either be implanted and allowed to develop, therefore creating an individual, or the embryo can be used as a source of totipotent/pluripotent stem cells. Pluripotent stem cells cannot differentiate into extra- embryonic stem cells, which form the placenta. Pre-1950s: cloning of plants through cutting and grafting (grapes, seedless oranges). 1950s: research in frogs shows scientists that cells from an early-stage embryo can be dissociated from the embryo and form identical animals (identical twins, triplets, etc. 1962: a frog was successfully cloned from adult intestinal cells. 1996: dolly became the first cloned mammal from adult cells. The udder cell of an adult sheep was fused with an enucleated egg.

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