01:119:150 Lecture 10: Genetically Modified Plants and Animals
Document Summary
Get access
Related Documents
Related Questions
View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
IV, V, I, II, III |
III, II, IV, V, I |
III, IV, V, I, II |
II, III, V, IV, I |
I, II, IV, III, V |
Flag this Question
Plasmids (or vectors) are important in biotechnology becausethey are
a vehicle for the insertion of recombinant DNA intobacteria. |
surfaces for respiratory processes in bacteria. |
recognition sites on recombinant DNA strands. |
surfaces for protein synthesis in eukaryotic recombinants. |
proviruses incorporated into the host DNA |
Flag this Question
Plasmids are put into bacterial cells by
restriction enzymes |
DNA ligase |
binding of cohesive sticky ends |
transformation |
Flag this Question
Restriction enzymes usually
cut donor DNA evenly so smooth edges result |
cut donor DNA but do not affect plasmids |
make staggered cuts at specific sequences in DNA in both donorDNA and plasmid |
are used in ligating plasmids into bacterial host cells |
more than one of the above |
Flag this Question
After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
Restriction enzyme |
DNA Ligase |
Reverse transcriptase |
DNA polymerase |
Helicase |
Flag this Question
It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
All organisms have ribosomes. |
All organisms have the same genetic code. |
All organisms are made up of cells. |
All organisms have similar nuclei. |
All organisms have transfer RNA. |
Flag this Question
Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
cut the plasmid with restriction enzyme X and insert thefragments cut with Y into the plasmid. |
cut the plasmid with enzyme X and then insert the gene into theplasmid. |
cut the DNA again with restriction enzyme Y and insert thesefragments into the plasmid cut with the same enzyme. |
cut the plasmid twice with restriction enzyme Y and ligate thetwo fragments onto the ends of the human DNA fragments cut withrestriction enzyme X. |
insert the fragments cut with X directly into the plasmidwithout cutting the plasmid. |
Flag this Question
Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
They are used to make proteins using a cloned gene. |
They contain a promotor. |
They are the first plasmid type used to clone a gene. |
They contain a terminator. |
More than one of the above is false. |
Flag this Question
What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
If the eukaryotic gene contains introns the bacteria will notremove them and the resulting amino acid sequence will be differentthat that made by a eukaryote. |
The bacteria may not fold the protein correctly. |
The bacteria may degrade the protein. |
All of the above are potential problems. |
Flag this Question
Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
True |
False |
Flag this Question
Question 111 pts
Gene cloning is used to do all of the following except
Make insulin |
Making genetically identical animals (e.g. Dolly thesheep) |
Make vaccines |
Perform Gene Therapy |
Making genetically engineered plants |
Flag this Question
In your
View/perform/read ALL THREE of the following prior to answeringthe questions.
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Stepsin Cloning a Gene (Links to an external site.)
http://www.discoverbiotech.com/wiki/-/wiki/Main/Applications ofCloning (Links to an external site.)
http://www.wiley.com/college/boyer/0470003790/animations/cloning/cloning.htm(Links to an external site.)
Skip to question text.
From the list below, which of the following is the most logicalsequence of steps for splicing foreign DNA into a plasmid andinserting the plasmid into a bacterium?
I. Transformbacteria with recombinant DNA molecule
II. Cutthe plasmid DNA using restriction enzymes
III. Extractplasmid DNA from bacterial cells
IV. Hydrogen-bondthe plasmid DNA to nonplasmid DNA fragments
V. Useligase to seal plasmid DNA to nonplasmid DNA
IV, V, I, II, III |
III, II, IV, V, I |
III, IV, V, I, II |
II, III, V, IV, I |
I, II, IV, III, V |
Flag this Question
Plasmids (or vectors) are important in biotechnology becausethey are
a vehicle for the insertion ofrecombinant DNA into bacteria. |
surfaces for respiratoryprocesses in bacteria. |
recognition sites onrecombinant DNA strands. |
surfaces for protein synthesisin eukaryotic recombinants. |
proviruses incorporated intothe host DNA |
Flag this Question
Plasmids are put into bacterial cells by
restriction enzymes |
DNA ligase |
binding of cohesive stickyends |
transformation |
Flag this Question
Restriction enzymes usually
cut donor DNA evenly so smoothedges result |
cut donor DNA but do notaffect plasmids |
make staggered cuts atspecific sequences in DNA in both donor DNA and plasmid |
are used in ligating plasmidsinto bacterial host cells |
more than one of theabove |
Flag this Question
After combining DNA fragments in a cloning experiment, ___ isused to covalently join the DNA segments.
Restriction enzyme |
DNA Ligase |
Reverse transcriptase |
DNA polymerase |
Helicase |
Flag this Question
It is theoretically possible for a gene from any organism tofunction in any other organism. Why is this possible?
All organisms haveribosomes. |
All organisms have the samegenetic code. |
All organisms are made up ofcells. |
All organisms have similarnuclei. |
All organisms have transferRNA. |
Flag this Question
Skip to question text.
Assume that you are trying to insert a gene into a plasmid andsomeone gives you a DNA sample cut with restriction enzyme X. Thegene you wish to insert from the given sample has sites on bothends for cutting by restriction enzyme Y. You have a plasmid with asingle site for Y, but not for X. Your strategy should be to
cut the plasmid withrestriction enzyme X and insert the fragments cut with Y into theplasmid. |
cut the plasmid with enzyme Xand then insert the gene into the plasmid. |
cut the DNA again withrestriction enzyme Y and insert these fragments into the plasmidcut with the same enzyme. |
cut the plasmid twice withrestriction enzyme Y and ligate the two fragments onto the ends ofthe human DNA fragments cut with restriction enzyme X. |
insert the fragments cut withX directly into the plasmid without cutting the plasmid. |
Flag this Question
Which of the following is/are false in regard to expressionplasmids (also called expression vectors)?
They are used to make proteinsusing a cloned gene. |
They contain a promotor. |
They are the first plasmidtype used to clone a gene. |
They contain aterminator. |
More than one of the above isfalse. |
Flag this Question
What is NOT a potential problem(s) associated with usingbacteria containing a cloned eukaryotic gene (e.g. a human gene) toproduce a functional protein?
If the eukaryotic genecontains introns the bacteria will not remove them and theresulting amino acid sequence will be different that that made by aeukaryote. |
The bacteria may not fold theprotein correctly. |
The bacteria may degrade theprotein. |
All of the above are potentialproblems. |
Flag this Question
Cloning allows for production of proteins in much larger amountsthan occurs in the cells from which the gene is isolated.
True |
False |
Flag this Question
Question 111 pts
Gene cloning is used to do all of the following except
Make insulin |
Making genetically identicalanimals (e.g. Dolly the sheep) |
Make vaccines |
Perform Gene Therapy |
Making genetically engineeredplants |
Flag this Question
In your