01:119:115 Lecture 17: DNA Technology
17 Jan 2019
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Document Summary
=> there is often not enough dna to run many tests. Pcr can produce billions of copies in a short period of time. Pcr helps study dna and read small samples. Components for pcr dntps = dna nucleotides (a,t,g,c) Primers = short (15-20 bp) dna fragments with specific sequences. Taq polymerase = dna polymerase from thermus aquaticus, bacteria from hot springs in yellowstone. Hydrogen bonds are weak and break at high temperature. One primer must be created for each dna strand. 40 c to 65 c to attach primers specifically to 3" side of the. 72 c, taq dna polymerase is used to replicate dna strand. Small fragments move far, big fragments stay close. Uses pcr to make lots of copies prior to sequencing. Primers = short segments with know 3" ends. 4 dideoxy ribonucleotides = labeled with florescent molecules. Used to identify the variety of different species that comprise a community.
