01:119:115 Lecture Notes - Lecture 34: Reverse Transcription Polymerase Chain Reaction, Spectrogram, Recombinant Dna
5 Apr 2016
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Document Summary
New strand sequence starts at 3" end of primer. Because there is no free oh the strand can not elongate. Generates a set of labeled strands of different lengths labeled by color. G tube: all four dntps, ddgtp and dna polymerase. A tube all four dntps, ddatp and dna polymerase. Plasmids-small usually circular pieces of dna that can replicate on their own, not part of the bacteria"s genome- often these plasmids give antibiotic resistance. Palindromes: when both strands are read in the 5"-3" direction are characteristic: restriction enzyme cuts sugar-phosphate backbones, dna fragment added from another molecule cut by same enzyme. Base pairing occurs: dna ligase seals strands. Gel electrophoresis: polyacrylamide gel (agrose polysaccharaide, separates by size, electrical charge, and other physical properties, used in conjunction with other molecular techniques. Method to produce billions of copies in a short period of time. 3 step process that is repeated 30-40 times: denaturation (breaking up, annealing (cooling, extension (building the chain)
